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The configuration method is as follows:
1 M Tris-HCl (pH 7.4, 7.6, 8.0) component concentration 1 M Tris-HCl preparation volume 1L Preparation method 1. Weigh 121.1g Tris into a 1L beaker.
2. Add about 800ml of deionized water, stir well to dissolve.
3. Add the amount of concentrated HCl in the table below to adjust the required pH value.
The pH value of concentrated HCl7.
4 is about 70ml, 7.6, about 60ml, about 42ml, and 4. The solution is made up to 1L.
5. After autoclaving, store at room temperature.
Note: The pH value should be adjusted after cooling the solution to room temperature, because the pH value of the Tris solution varies greatly with temperature, and the pH value of the solution decreases by approximately 0.03 units for every 1°C increase in temperature.
1.5MTris-HCl (pH8.8) component concentration 1.5MTris-HCl preparation volume 1L Preparation method 1. Weigh 181.7g Tris into a 1L beaker Chemicalbook.
2. Add about 800ml of deionized water, stir well to dissolve.
3. Adjust the pH value to 8.8 with concentrated HCl.
4. Make the solution volume up to 1L.
5. After autoclaving, store at room temperature.
Note: The pH value should be adjusted after cooling the solution to room temperature, because the pH value of the Tris solution varies greatly with temperature, and the pH value of the solution decreases by approximately 0.03 units for every 1°C increase in temperature.
Tris buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many important uses.
Tris is used for protein crystal growth under different pH conditions. The low ionic strength of Tris buffer can be used for the formation of the intermediate fiber of C.elegans nuclear lamin (lamin).
Tris is also one of the main components of protein running buffer. In addition, Tris is also an intermediate for the preparation of surfactants, vulcanization accelerators and some drugs. Tris is also used as a titration standard.
Dangerous goods mark Xi
dangerous category code 36/37/38
Safety instructions 26-36-37/39-24/25-23
WGKGermany 2
RTECS number TY2900000
F 3
TSCA YesHazardClassIRRITANT
Customs Code Chemicalbook code 29221980
Toxic Substances Data 77-86-1 (HazardousSubstancesData)
Toxicity LD50orallyinRabbit:>5000mg /kgLD50dermalRat>5000mg/kg
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